| dc.description.abstract | Developing new treatment method using natural products to prevent
different diseases including microbial activity is necessary. The aim of this study
was to investigate the in vitro antimicrobial activity of individual leaf extracts and
their combinations of Piper lolot and Piper betle, which were collected in Southern
Vietnam. Crude ethanol extract from dried Piper lolot, Piper betle leaves ( nonvolatile
extract) and aqueaous extract from fresh leaves (volatile extract) produced
by sonication and microwave hydrodistillation, were examined against pathogenic
microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa,
Salmonella typhimurium, and Escherichia coli. Screening for antimicrobial activity
was performed using agar disc diffusion and micro-titer broth dilution methods.
The combined effect of nine ratios of two plant’s extracts was also evaluated using
checkerboard method. The study shows that most of combined extracts have
synergism effects. The practical issues of each extraction method and antimicrobial
activity screening of 6 different combinations in 9 ratios of plant extracts were
discussed in this study.
Keywords: antimicrobial activity, Piper lolot, Piper betle, leaves extract,
essential oil, sonication, microwave hydrodistillation, disc diffusion, minimum
inhibitory concentration, combination. | en_US |
