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dc.contributor.authorNghia, Le Tri
dc.date.accessioned2015-10-05T01:39:26Z
dc.date.accessioned2018-05-31T01:46:31Z
dc.date.available2015-10-05T01:39:26Z
dc.date.available2018-05-31T01:46:31Z
dc.date.issued2014
dc.identifier.urihttp://10.8.20.7:8080/xmlui/handle/123456789/1561
dc.description.abstractAspergillus flavus and Aspergillus parasiticus are the common aflatoxin producing species that usually infect foodstuff in their production line from the field to the storage place such as peanut, corn, cereal, etc especially in tropical country like Vietnam. Aflatoxins which are considered as derived secondary metabolites assigned as a group of mycotoxins produced by several species of the Aspergillus spp are potent hepatotoxins, immunosuppression, carcinogen that lead to mortality or reducing the productivity of farm animals. There has been a demand for effective method to detect these two species on dried food. In previous study, a multiplex PCR method were designed to improve the detection process of A. flavus and A. parasiticus and that method showed high sensitivity and specificity by being applied on artificially infected dried peanut. In this study, that multiplex PCR method would be evaluated by testing the presence of A. flavus and A. parasiticus on natural dried peanut kernels. On this purpose, the presence of A. flavus and A. parasiticus on the collected peanut from the market was determined using two method, the conventional culturing method in Institute of Hygiene and Public Health (IHPH) and the mentioned multiplex PCR. The efficiency of multiplex PCR method would be evaluated by comparing fungi detection result of two methods using appropriate statistical tests. The result showed that 54% results from PCR method was the same with culturing method, and after fungal enrichment by incubating peanut kernels with distilled water overnight, this percentage increased to 76% which was suggested not to be significantly different with culturing method. Therefore, this multiplex PCR method was more advanced than the culturing method in detection of A. flavus and A. parasiticus on foodstuff. Key words Aspergillus flavus Aspergillus parasiticus Culturing method Dried peanut kernels IHPH Multiplex PCR methoden_US
dc.description.sponsorshipDr. Nguyen Thi Hueen_US
dc.language.isoen_USen_US
dc.publisherInternational University HCMC, Vietnamen_US
dc.relation.ispartofseries;022001275
dc.subjectAspergillus flavusen_US
dc.titleEvaluation of the optimal multiplex PCR method for the detection of Aspergillus flavus and Aspergillus paraciticus in dried peanuts +CDen_US
dc.typeThesisen_US


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