| dc.description.abstract | Staphylococcus aureus (S. aureus) is a considerably pathogenic hazard in
human because it was the fifth rank nosocomial infectious cause. It is also one
of the most commonly isolated organisms in infectious diseases in Vietnam. S.
aureus isolates from intensive care units across the country and from blood
culture isolates worldwide are increasingly resistant to a greater number of
antimicrobial agents. The emergence of antibiotic resistance is driven by
antibiotic use, along with other factors such as the spread and fate of bacteria,
resistance genes and antibiotics, as well as the behavioral and medical
activities of people. The contribution of these factors produces the problem and
the difficulty in reserving it.
In this study, 2D-PAGE was set up and optimized to figure out potential protein
markers in antibiotic resistance and would support further analysis of their
roles in antibiotic resistance mechanism in future studies.
This study was to be done via running 2D-PAGE and then comparing protein
profiles between flouroquinolone (FQ) sensitive S. aureus ATCC 29213 to the
in-vitro induced FQ resistant S. aureus ATCC 29213.
Although 2D-PAGE is quite costly, this technique is powerful thus widely used in
many laboratories all over the world. With this work, 2D-PAGE technique has
already been established, optimized and ready to serve further research at our
institution.
Key word: S. aureus, 2D-PAGE, flouroquinolone, resistant | en_US |
