| dc.description.abstract | In this study, the influence of fluoxetine, a SSRI antidepressant, on the
conformation of 1,2-dioleoyl-sn-glycero-3-phosphocholine(DOPC) bilayer was
investigated using FTIR to provide insights into the drug’s behavior and mode of
action. Infrared spectroscopic bands assigned to the CH2 stretching and PO2
-
stretching vibrations revealed that fluoxetine at a concentration of 1.14 mM
influences both hydrophobic and hydrophilic parts of DOPC bilayers. In addition,
in the presence of cholesterol at several concentration from low to high (10
mol%, 20 mol%, 28 mol%) in DOPC liposome, there were the lipid
conformational changes in interacting with the drug. The phosphate and alkyl
chain regions were demonstrated for the decrease of lipid conformation in pure
DOPC liposomes and DOPC-Cholesterol 10 mol%. However, at higher cholesterol
concentration, the lipid conformation remains unchanged. Suggesting that higher
concentration of cholesterol suppresses the incorporation of fluoxetine into DOPC
bilayers. These results showed that the interactions of fluoxetine and lipid
membrane play a significant role in the drug partitioning and this, in turn, affects
the drug action. The study improves our understanding about drug action at
molecular level and could aid in the rational design of antidepressant drugs.
Keywords:
Antidepressant
FTIR
Lipid conformation
Fluoxetine
Cholestero | en_US |
