| dc.description.abstract | In other to synchronize the pool of collected porcine oocytes for in vitro maturation
(IVM) to produce good mature oocyte supporting for in vitro fertilization (IVF) and
enhance the developmental competence of embryo, dibutyryl cAMP (dbcAMP) was
added to the IVM medium. This study investigated the effect of dbcAMP on two
parameters, meiotic competence, and cumulus expansion rate. Fully grown porcine
oocytes were matured in standard IVM, bicarbonate-buffered TCM-199
supplemented with 10% (v/v) fetal bovine serum, 0.1 IU/ml human chorionic
gonadotropin, 0.1 mg/ml sodium pyruvate, and 10% (v/v) porcine follicular fluid as
the control group. Two concentrations of dbcAMP, 0.5mM, and 1mM were added to
the standard IVM during the first 20-22 hours of culturing for transient blocking of
meiosis as +dbcAMP 0.5mM and +dbcAMP 1mM group respectively. We also
established an IVF system using three kinds of medium Fert-TALP, Pig-FM, and
mTBM. The group of +dbcAMP 1mM showed the best effect on the cumulus
expansion rate (68.43%) compared with control group without dbcAMP and
+dbcAMP 0.5 mM (42.01% and 50.49%, respectively). However, there was no
significant difference in the meiotic competence between groups (P>0.05). After the
establishment of IVF, we can obtain normal pronucleus (monospermy) in some
embryo with decondensed chromatin at 6-10 hours after IVF, condensed chromatin
at 14-16 hours after IVF and metaphase at 18-20 hours after IVF. The abnormal
fertilization, polyspermy and sperm arrested, was also observed. These findings
make a contribution to a better understanding of the effect of dbcAMP to pig oocyte
maturation which is essential to develop an in vitro fertilization system with the
higher normal embryo rate.
Keywords: porcine, oocyte, in vitro maturation, in vitro fertilization, cAMP, cumulus
expansion | en_US |
