| dc.description.abstract | Recently, betalains have gained the attention as natural food colorants because of their high anti-oxidative and free radical scavenging activities. This study focused on the process to induce the root formation from explants of Beta vulgaris L. - the main source of betalains by auxin supplementation and how to maximize the betalains amount produced in shake flask culture. IAA (1mg/L) was the best treatment to induce more roots from root explants, hypocotyl and cotyledon explants effectively rooted with NAA (0.5mg/L) and NAA (1mg/L) respectively. In shake culture, Beta vulgaris L. roots at 7g/L inoculum density reached peak of betalains (0.34mg/g FW) after 4-week cultivation at 7g/L inoculum; sucrose concentration at 20g/L and 30g/L show little significant difference in growth ratio as well as pigment biosynthesis. The addition of 1mg/L IAA remarkably increased growth of Beta vulgaris L. roots and the highest record of betalains yield was 0.53mg/g FW after 2-week cultivation.
Keywords: Adventitious root, auxins, Beta vulgaris, betalains and food colorant. | en_US |
