Cloning and expression of aprE gene encoding NATTOKINASE in Bacillus subtilis

Abstract

There is an increasing risk of thromboembolism all over the world, the study of its therapeutic treatment is a necessity. Several studies and treatments were published worldwide. Nattokinase which is isolated from boiled soybean fermented food Natto of Japanese have been known as a treatment of thromboembolia. Nattokinase were extracted and commercialized as a functional food, but its price is still high for Vietnamese costumers. The aim of this study is set up an over expression of aprE gene encoding Nattokinase in Bacillus subtilis. A recombinant plasmid pAX01- aprE is transformed into Bacillus subtilis to over-express high content of recombinant protein Nattokinase. From 4 different samples of Natto, Bacillus natto, which has high fibrin degradation activity, was isolated and screened. Applied the basic genetic methods, the recombinant plasmid were successfully created. Key words: natto, nattokinase, fibrin degradation, Bacillus subtilis, Bacillus natto, pAX01- aprE