| dc.description.abstract | The surface binding of polyamidoamine (PAMAM G2.0, G3.0, G4.0, ) dendrimers with Fucoidan to reduce the toxicity have been analyzed by multiple spectroscopic methods, dynamic Light Scattering (DLS) to determine the size, Zeta Potential analysis is for determining the surface charge of nanoparticles in solution and FDA / EB method for evaluating cytotoxicity of the copolymer conjugation on the stability and morphology to determine the best ratio for PAMAM dendrimer and Fucoidan. Structural analysis showed that drug–polymer conjugation occurs mainly via electrostatic interaction on surface.
Afterthat, 4.65% Letrozole (LET) based on the mass of total PAMAM dendrimer G3.0 – LET – Fucoidan was encapsulated. LET forms more stable conjugate with PAMAM dendrimer G3.0 – Fucoidan at ratio 1:2 which is based on the result of DLS showed the size of the PAMAM morphology upon drug encapsulation was 101.133 ± 0.289 nm, Zeta Potential measurement of drug – copolymer was -36.9 ± 0.265 mV permit that the linked between them was stable. The dendrimer PAMAM – Fucoidan system encapsulate LET, the %EE yield of LET is 91.84%. This indicates that the drug loaded inside the system is very effective to carry hydrophobic anticancer drug .
In-vitro test, LET encapsulated in PAMAM G3.0 coated Fucoidan could release approximately 70.121%, 73.71% at pH=74 and pH=5.5 after 5 days, respectively. The obtained resulted possibly offered a great potential of the nanocarriers which may be utilized in delivering another anticancer drugs or dual drugs for chemotherapy in future. | en_US |
