| dc.description.abstract | Pectinase and cellulase are also well-known terms for commercial enzyme preparation that have wide application, however, the high cost used in the importation of industrial extracellular enzymes has led to the high cost of finished industrial products. This thesis was aimed to study conditions to produce pectinase and cellulase by Aspergillus niger (A.niger) in molasses including the sucrose concentration in molasses, inoculum size, substrates concentration, incubation time to temperature in the purpose of reducing production expense. Enzymes activities were determined by 3, 5-dinitrosalisylic acid (3,5-DNS) assay. Then, those were applied on mucilage of cocoa with the ratio 1:1 (the same volume of pectinase and cellulase for each treatment) to investigate the optimal concentration of enzymes and duration for treatment to achieve the highest amount of removal of cocoa’s mucilage. As the result, the highest pectinase and cellulase activity were 315.97 ± 0.34 and 64.95 ± 0.05 (U/mL) achieved at 2oBrix and 0.8oBrix of molasses concentration for 96 and 120 hours with 0.5% of pectin and 0.4% of CMC as substrate. Both enzymes were similarly obtained with initial pH 4.5, 106 cells/mL, 1% of (NH4)2SO4 as nitrogen source at 35oC. For cocoa treatment, the highest amount of removed mucilage was 98.25%, with 2% treated enzymes incubated at 35oC for 2 days (48 hours) and was 3.6 times higher than these amount of the control.
Key words:
Aspergillus niger, Cellulase, Molasses, Mucilage of cocoa, Pectinase | en_US |
