Study On Cytotoxicity And Phytochemical Composition Of The Ethyl Acetate Extract From Macrosolen Tricolor

Abstract

Some species of the Macrosolen genus have been shown to possess important biomedical properties, including cytotoxic activity. In this study, we examined the cytotoxic effect of ethyl acetate extract from Macrosolen tricolor in human cancer cell lines of MDA-MB-231 and RD by using MTT assay. The most cytotoxic fraction MTE.I (IC50 value of 4.00 µg/mL and 5.53 µg/mL with MDA-MB-231 and RD cell lines, respectively) was applied to Column Chromatography to afford two pure compounds MTE01 and MTE02. By using nuclear magnetic resonance experiments (1D and 2D NMR), the structures of MTE01 and MTE02 were verified as 4α,5α- dihydroxybisabola-2,10-diene-9-one (bisacurone A) and 3-O--Dglucopyranosylstigmast-5-ene (β-Sitosterol 3-O--D-glucoside), respectively.