| dc.description.abstract | The striped catfish (Pangasianodon hypophthalmus) (Sauvage, 1878), locally
known as tra catfish, is one of the iconic species for aquaculture development in
Vietnam. The striped catfish genome sequenced in 2018 was the foundation for
assisting broodstock selection, conservation and fingerprinting. This study
identified 145 microsatellite markers based on the published genomic sequences
of the striped catfish. By adopting direct PCR method and 4% agarose gel
electrophoresis, 63 polymorphic markers with specific PCR amplicons were
screened and selected for multiplexing PCR. This study also succeeded in
developing a multiplex-PCR set with 10 microsatellite markers which was
experimented to evaluate the polymorphism on 12 wild striped catfish individuals.
The result analyzed by capillary electrophoresis system with laser-induced
fluorescence detection demonstrated the mean number of alelles of 7 alelles per
locus, the mean polymorphic information content (PIC), the mean observed and
expected heterozygosity of 0.708, 0.742 and 0.775, respectively. As a result, this
newly developed set of microsatellite markers was informative for further
investigations in the striped catfish molecular genetics. | en_US |
